Discriminatory power of RAPD, PCR-RFLP and southern blot analyses of ureCD or ureA gene probes on Helicobacter pylori isolates.

نویسندگان

  • Stella Smith
  • Franck Cantet
  • Fabrice Angelini
  • Armelle Marais
  • Francis Mégraud
  • Ekkhehard Bayerdöffer
  • Stephan Miehlke
چکیده

The genetic diversity of 33 Nigerian Helicobacter pylori isolates were studied using RAPD, PCR-RFLP and Southern blot analysis of ureA or ureCD gene probes. RAPD was able to distinguish the following number of isolates using the primers 3880: 5'-AAGAGCCCGT-3' (28), 3881 :5'-AACGCGCAAC-3' (33) and OPH8 :5'-GAAACACCCC-3' (25). Southern blot analysis using the ureCD probe was also able to distinguish the 12 isolates tested into ten different patterns. The PCR-RFLP technique distinguished all 33 isolates into six types. In conclusion, considering typeability, discriminatory power, and convenience, RAPD with the 3881 primer was considered the most useful technique.

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عنوان ژورنال:
  • Zeitschrift fur Naturforschung. C, Journal of biosciences

دوره 57 5-6  شماره 

صفحات  -

تاریخ انتشار 2002